GENIPOL Genomic tools for bio-monitoring of pollutant coastal impact

Contract EVK3-CT-2001-00057 EU 5th Framework Energy, Environment & Sustainable Development Program…….......... 1ST November 2001-31st October 2004
Key Action 3. Sustainable Marine ecosystems…Monitoring and managing processes of the coastal zone.

Project aims:


To develop new versatile diagnostic tools that will identify adverse biological effects of environmental pollutants on fish in coastal and marine environments, to allow coastal managers to detect trends in the state of the coastal ecosystem and assess the sustainability of on-going development, thus permitting the coexistence of a stable coastal environment and a nearby highly developed industrial society.

Scientific approach:


1. To employ FUNCTIONAL GENOMIC and PROTEOMIC expression analysis to identify a suite of diagnostic gene responses to pollutant exposure in two sentinel inshore fish species …................ ...........................European Flounder (P.flesus) and Striped Sea Bream (L. mormyrus )

 


2. To develop practical monitoring tools:

i) cDNA miniarrays for qualitative diagnosis of adverse health effects and pollutant class identification
ii) quantitative assays (based upon Q RT-PCR and ELISA techniques) for selective impact assessment and long term trends monitoring..

3. To validate these tools in field situations through an intercomparison exercise.


4. To produce an effects database for community use.

     

 

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