Genomic tools for bio-monitoring of pollutant coastal impact
EU 5th Framework Energy, Environment & Sustainable Development
November 2001-31st October 2004
Key Action 3. Sustainable Marine ecosystems
managing processes of the coastal zone.
To develop new versatile diagnostic tools that will
identify adverse biological effects of environmental pollutants
on fish in coastal and marine environments, to allow coastal managers
to detect trends in the state of the coastal ecosystem and assess
the sustainability of on-going development, thus permitting the
coexistence of a stable coastal environment and a nearby highly
developed industrial society.
1. To employ FUNCTIONAL GENOMIC and PROTEOMIC expression
analysis to identify a suite of diagnostic gene responses to pollutant
exposure in two sentinel inshore fish species
(P.flesus) and Striped Sea Bream (L.
2. To develop practical monitoring tools:
i) cDNA miniarrays
for qualitative diagnosis of adverse health effects and pollutant
ii) quantitative assays (based upon Q RT-PCR and ELISA techniques)
for selective impact assessment and long term trends monitoring..
3. To validate
these tools in field situations through an intercomparison exercise.
4. To produce an effects database for community use.