PROGRAM

How the program fits together

WORKPACKAGES

 

Wpg#

Activity

Partners*

   

1

Experimental pollutant exposures

IOLR

IOA

 

2

Flounder, bream cDNA libraries, flounder cDNA microarrays

IOLR

IOA

UB

3

Identification pollutant responsive genes

IOLR

IOA

UB

4

Identification pollutant responsive proteins

CPA

   

5

Preparation diagnostic cDNA miniarrays

IOLR

IOA

UB

6

Development of RT-PCRs and ELISAs

IOLR

IOA

 

7

QA/QC of diagnostic methods and testing

IOLR

ALL

 

8

Expression data archiving

IOA

   

9

Project management, integration, reporting

IOLR

ALL

 

DELIVERABLES

Deliverable

No.

Deliverable title

Delivery date

Nature

Dissemin-ation level

1

Hepatic extracts of sampled and experimentally exposed fish suitable for 2DE, SHH cloning, reverse Northern hybridization, RT-PCR and ELISA

2 - 24

Re

CO

2

Cloned fish liver expressed partial cDNAs

6

Pr

CO

3

cDNA microarrays on glass slides

12

Da

CO

4

Sequenced pollution affected hepatic cDNAs

16 - 30

Da

CO

5

Identification of differentially-expressed protein spots

4 - 25

Me

CO

6

Partial sequencing of each of the identified proteins by MS

6 - 30

 

CO

7

cDNA microarrays of pollution affected genes

34

 

1PU

8

Established RT-PCRs for selected biomarker transcripts

8 - 30

Me

1PU

9

Established ELISAs for selected biomarker transcripts

8 - 30

 

1PU

10

Evaluated within-samples variations in comparison to among-samples differences

35

 

1PU

11

Delivery of intermediate products (clones and proteins)

2 - 34

 

CO

12

Exchange of intermediate results for analysis (sequences, patterns of expression)

4 - 34

 

CO

13

Reports and publications

36

Re

PU

RESULTS

Please see Results and Publications pages for latest public findings